![]() Ĭurrently, the most popular methods are based on droplet-based barcoding: encapsulating single cells with oligonucleotide barcodes in nanolitre droplets. Single-cell transcriptomics has been already used in very diverse animal groups, including sponges, cnidarians, placozoans, ctenophores, planarians, nematodes, arthropods, ascidians, and extensively in vertebrates. Using these methods, scientists have already profiled a broad taxonomic range of different animals, classified their cell types, profiled their gene expression patterns, and begun to reconstruct their cell differentiation lineages. ![]() From the sequencing of a single cell in 2009, we have seen year-on-year exponential increases in the number of cells that can be sampled by scRNA-seq. The mRNAs of each cell are indicative of the cell type or state and allow biological questions to be addressed at a new level of integration and detail. For instance, single-cell transcriptomics (or scRNA-seq) allows the measurement of the expression of thousands of mRNAs from potentially hundreds of thousands of individual cells. The cell is the fundamental unit of biological systems, and studying thousands of them individually allows reconstruction of the cellular diversity and dynamics formerly blended into bulk tissue samples. Biology is undergoing a paradigm shift due to the introduction of single-cell sequencing methods.
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